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anti erk1 2  (Cell Signaling Technology Inc)


Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
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    Structured Review

    Cell Signaling Technology Inc anti erk1 2
    Anti Erk1 2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bad/Phospho-Bad+Antibody+Sampler+Kit/pm41898298-93-47-48
    Average 93 stars, based on 15 article reviews
    anti erk1 2 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Targeting the MARCH5-MFN2 axis to enhance mitochondrial fusion and sensitize multiple myeloma cells to venetoclax
    Article Snippet: Protein samples (30 μg per lane) were resolved on 10% SDS-PAGE gels and subsequently transferred onto nitrocellulose membranes using the Trans-Blot Turbo Transfer System (Bio-Rad Laboratories, Hercules, CA, USA). .. Membranes were then blocked and incubated with the following primary antibodies: MARCH5 (#PA5-25584) (Thermo Fisher Scientific, Waltham, MA, USA); MFN2 (# 9482S), FLAG(#F3165), MFF(#84580S), DRP1 (#14647S), UBIQUITIN (#3933S), PARP (#9532), CASPASE 3 (#9665P), CASPASE 8 (#9746P), BAD (#9239), BIK (#4592), BAK(#12105), BID(#2002), BIM (#2933), NOXA (#14766), NDUFS1 (#70264), NDUFAB1 (#71814), UQCRFS1 (#95231), COX1 (#55159), COX IV (#4850) were from Cell Signaling Technology (Denvers, MA, USA); PUMA (SC-374223) was from Santa Cruz Biotechnology (Dallas, TX, USA). γ-Tubulin (T6557; Sigma-Aldrich, St. Louis, MO, USA), GAPDH (#5174; Cell Signaling Technology, Denvers, MA, USA) and α-TUBULIN (#2125S; Cell Signaling Technology, Denvers, MA, USA) were used as loading controls. .. Protein bands were visualized using the Clarity Western ECL Substrate (Bio-Rad Laboratories, USA), and chemiluminescence signals were quantified using the UVITEC Imaging System (Cleaver Scientific, UK).

    Article Title: Obacunone alleviated the development of polycystic ovary syndrome via inhibiting STAT3 phosphorylation
    Article Snippet: .. Next, the membranes were incubated overnight at 4 °C with the following primary antibodies: cleaved caspase-3 (1:500, #9661, Cell Signaling Technology, USA), cleaved caspase-9 (1:1000, MA5-53678, Thermo Fisher, USA), Bad (1:1000, #9239, Cell Signaling Technology), Bcl-xl (1:1000, #2764, Cell Signaling Technology), IL-10 (1:1000, #12163, Cell Signaling Technology), IL-18 (1:1000, #67775, Cell Signaling Technology), TNF-α (1:500, ab183218, Abcam, UK), IL-1β (1:2000, 26048-1-AP, Proteintech, Wuhan, China), Bcl-2 (1:500, ab194583, Abcam), Bax (1:1000, #2772, Cell Signaling Technology), IL-6 (1:2000, 21865-1-AP, Proteintech), p-STAT3 (1:500, ab32143, Abcam) and STAT3 (1:1000, 10253-2-AP, Proteintech). .. GAPDH (1:2000, 10494-1-AP, Proteintech) served as an endogenous control.

    Article Title: Carboxypeptidase D deficiency causes hearing loss amenable to treatment
    Article Snippet: .. The membrane was then blocked in 5 % BSA for 1 hour and incubated overnight at 4°C with primary antibodies ERK1/2 (Cell signaling Technology, USA, Cat# 9532S), pERK1/2 (Cell signaling Technology, USA, Cat# 5625S), AKT (Cell signaling Technology, USA, Cat# 9272S); pAKT (Cell Signaling Technology, USA, Cat# 4060S), BAD (Cell signaling Technology, USA, Cat# 9239S); pBAD (Cell Signaling Technology, USA, Cat# 4366S/9291S), CREB (Cell signaling Technology, USA, Cat# 4820S); pCREB (Cell signaling Technology, USA, Cat# 9198S), and Caspases-3 (Cell signaling Technology, USA, Cat# 14220S) each diluted at 1:1000 in 5 % BSA + TBST (TBS with 0.5% Tween). .. The next day, the membrane was washed and incubated with anti-rabbit or anti-mouse peroxidase-conjugated secondary antibody (1:3000) and developed using West Pico Super-Signal ECL substrate (Thermo Scientific, USA, 37069).

    Ubiquitin Proteomics:

    Article Title: Targeting the MARCH5-MFN2 axis to enhance mitochondrial fusion and sensitize multiple myeloma cells to venetoclax
    Article Snippet: Protein samples (30 μg per lane) were resolved on 10% SDS-PAGE gels and subsequently transferred onto nitrocellulose membranes using the Trans-Blot Turbo Transfer System (Bio-Rad Laboratories, Hercules, CA, USA). .. Membranes were then blocked and incubated with the following primary antibodies: MARCH5 (#PA5-25584) (Thermo Fisher Scientific, Waltham, MA, USA); MFN2 (# 9482S), FLAG(#F3165), MFF(#84580S), DRP1 (#14647S), UBIQUITIN (#3933S), PARP (#9532), CASPASE 3 (#9665P), CASPASE 8 (#9746P), BAD (#9239), BIK (#4592), BAK(#12105), BID(#2002), BIM (#2933), NOXA (#14766), NDUFS1 (#70264), NDUFAB1 (#71814), UQCRFS1 (#95231), COX1 (#55159), COX IV (#4850) were from Cell Signaling Technology (Denvers, MA, USA); PUMA (SC-374223) was from Santa Cruz Biotechnology (Dallas, TX, USA). γ-Tubulin (T6557; Sigma-Aldrich, St. Louis, MO, USA), GAPDH (#5174; Cell Signaling Technology, Denvers, MA, USA) and α-TUBULIN (#2125S; Cell Signaling Technology, Denvers, MA, USA) were used as loading controls. .. Protein bands were visualized using the Clarity Western ECL Substrate (Bio-Rad Laboratories, USA), and chemiluminescence signals were quantified using the UVITEC Imaging System (Cleaver Scientific, UK).

    other:

    Article Title: Comparative DNA damage induced by eight nitrosamines in primary human and macaque hepatocytes
    Article Snippet: Supernatants were collected by centrifugation and the protein concentrations were determined using the Pierce BCA protein assay kit.

    Membrane:

    Article Title: Carboxypeptidase D deficiency causes hearing loss amenable to treatment
    Article Snippet: .. The membrane was then blocked in 5 % BSA for 1 hour and incubated overnight at 4°C with primary antibodies ERK1/2 (Cell signaling Technology, USA, Cat# 9532S), pERK1/2 (Cell signaling Technology, USA, Cat# 5625S), AKT (Cell signaling Technology, USA, Cat# 9272S); pAKT (Cell Signaling Technology, USA, Cat# 4060S), BAD (Cell signaling Technology, USA, Cat# 9239S); pBAD (Cell Signaling Technology, USA, Cat# 4366S/9291S), CREB (Cell signaling Technology, USA, Cat# 4820S); pCREB (Cell signaling Technology, USA, Cat# 9198S), and Caspases-3 (Cell signaling Technology, USA, Cat# 14220S) each diluted at 1:1000 in 5 % BSA + TBST (TBS with 0.5% Tween). .. The next day, the membrane was washed and incubated with anti-rabbit or anti-mouse peroxidase-conjugated secondary antibody (1:3000) and developed using West Pico Super-Signal ECL substrate (Thermo Scientific, USA, 37069).



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